Amplicon sequencing services enable the detection of low frequency variants, quantitative analysis of mixed populations, and scalable analysis of many samples with solutions including 16S and 18S amplicon sequencing. GENEWIZ Multiomics & Synthesis Solutions from Azenta Life Sciences offers amplicon-based next generation sequencing (NGS) of amplicons (PCR products) to validate/discover variations in specific genomic regions or screen clones. GENEWIZ Amplicon Sequencing Services employ the latest technologies to provide custom, in-depth solutions for amplicons ranging from 100 base pairs (bp) to >10,000 bp.
Applications for this service include antibody library screening, natural antibody repertoire profiling, disease genotyping, CRISPR mutation analysis, 16/18S gene sequencing, screening of molecular clones, and analysis of plasmid stocks.
Multiple Solutions to Answer Every Biological Question
Azenta's Recommendation | ||||
Amplicon Size | Applications | Library Preparation Type | Platform Configuration | |
100-250 bp |
|
Adapter ligation to |
Illumina 2x150 bp | |
250-550 bp |
|
Adapter ligation to full-length products |
Illumina 2x250 bp or 2x300 bp |
|
|
Fragmentation | Illumina 2x150 bp | ||
>550 bp |
|
Adapter ligation to full-length products |
PacBio Sequel | |
|
Fragmentation | Illumina 2x150 bp |
Amplicon sequencing is a targeted sequencing method that uses polymerase chain reaction (PCR) to selectively amplify specific DNA fragments or genomic regions of interest. Specific primers are designed to flank the target region and the resulting amplicons are then sequenced to determine the nucleotide sequence.
Targeted sequencing and amplicon sequencing both selectively sequence specific regions of the genome, rather than the whole genome. However, the difference between the two methods lies in how the regions of interest are selected and amplified.
Targeted sequencing amplifies through hybridization capture and uses probes or baits complimentary to the target region in order to selectively capture and enrich for specific genomic regions, such as genes, exons, and other functional regions of the genome. Targeted sequencing often offers greater coverage than amplicon sequencing, as the use of hybridization capture can help mitigate biases in the representation of certain genomic regions.
Amplicon sequencing amplifies DNA fragments using PCR. The target regions are defined by PCR primers designed to anneal specific sequences that flank the areas of interest, initiating the amplification process of the targeted DNA fragments. Amplicon sequencing is often more cost-effective than targeted sequencing as it does not involve the use of capture reagents.
A biopharma company required a partner for clinical trial support, with an immediate need for development and validation of a biomarker assay using next generation sequencing with a quick turnaround time. Read the case study to learn how this company was able to streamline clinical testing and accelerate the timeline of its phase 1/2 study.
This selection guide offers practical information about PCR + Sanger, qPCR, and NGS approaches to help you determine which assay best suits your project requirements, along with an interactive assay selection tool to aid your decision making.
Azenta accepts purified and unpurified amplicons, restriction digested material, fragmented DNA, and plasmids. For detailed sample submission requirements please visit our Sample Submission Guidelines.