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High-throughput gene expression (HT-GEx) screening is an RNA-Seq-derived approach we developed for optimal drug discovery and analysis, similar to DRUG-Seq. Unlike microarrays, our method offers rapid, unbiased, 3' gene expression screening, providing complete cell-to-data analysis of the transcriptome at a lower cost than standard RNA sequencing. Working directly from lysate, GENEWIZ High-Throughput Gene Expression Screening works directly from cell lysate, saving both time and money by removing the need for RNA purifications. Additionally, the method performs 3’ transcript tagging to reduce required sequencing depths.
High-Throughput Gene Expression Screening Applications
Drug Discovery
Compound Treatment Phenotyping
CRISPR Treatment Phenotyping
Cell Response Screening
HIGH-THROUGHPUT GENE EXPRESSION SCREENING WORKFLOW
High-Throughput Gene Expression Screening Assay Comparison
| Metric | HT-GEx Screening | mRNA-Seq | Total RNA-Seq | Iso-Seq |
| Reads/Sample | ~1-2M Reads | ~10-30M Reads | ~20-45M Reads | ~1-2M Reads |
| Min. Sample Size | 96/384 | 1 | 1 | 1 |
| Cost | $ | $$ | $$$ | $$$$ |
| Starting Material | Cell Lysate | >20ng Total RNA | >100ng Total RNA | >1ug Total RNA |
| Selection Method | Poly(A) + | Poly(A) + | rRNA - | Poly(A) + |
| Detection Level | 3' Tails of Genes | Poly(A) Transcript | All Transcript | Full-Length Poly(A) Transcript |
| Gene Expression | ||||
|
Variant Detection |
||||
| Alternative Splicing | ||||
| Transcriptome Assembly |
Suitable method | Preferred method | Not a suitable method
High-Throughput Gene Expression Screening Deliverables
Standard Deliverables
- FASTQ files
- Quality summary report
Advanced Analysis
(In addition to standard deliverables)
- UMI extraction, mapping, counting, and transformation
- Normalization
- Differential gene expression and clustering by compound/treatment
Custom bioinformatics analysis and reports are available. Please contact us about how we can customize the analysis to answer your biological question.
High-Throughput Gene Expression Screening Features & Benefits
Fast Turnaround Times
Starting at just 2 weeks for quick target discovery
High-Throughput Capacity
To rapidly scale up pilot or discovery projects
Superior Data Quality
Exceeding manufacturers’ benchmarks
High-throughput, cell-to-data solution for compound screening
Low-cost, unbiased coverage for use in selection of drug candidates
Receive detailed expression data for each well across multiple 96- or 384-well plates
Free technical consultation and customer support from Ph.D.-level project managers and lab scientists
Webinar Series | Advancing Transcriptomics: Gene Expression Screening, Single-Cell RNA-Seq, and Beyond
With this two-part webinar series, go beyond traditional transcriptomics and learn about the various NGS approaches available for gene expression analysis. In part 1, we take an in-depth look at various gene expression approaches, including RNA-Seq, single-cell RNA-Seq, digital spatial profiling, and more. In part 2, we explore the data generated from these approaches and how they can complement each other and confirm findings.
Tech Note | Achieving Phenotypic Profiling for Rapid Drug Discovery with High-Throughput Gene Expression Screening
High-throughput technologies are critical in performing phenotypic profiling for drug discovery applications. In this tech note, Azenta Life Sciences discusses the challenges associated with traditional approaches, such as microarrays and RNA sequencing, and offers an optimized assay to achieve high-quality phenotypic profiling at a reduced cost for rapid drug discovery.
NGS PLATFORMS
For information on our NGS platforms as well as recommended configurations of your projects, please visit the NGS Platforms page. Azenta does not guarantee data output or quality for sequencing-only projects.
GENEWIZ from Azenta Life Sciences
European GENEWIZ Headquarters
GENEWIZ Germany GmbH, Bahnhofstrasse 86, 04158 Leipzig | +49 (0)341 520 122-41