WHOLE PLASMID SEQUENCING: PLASMID-EZ

Whole plasmid sequencing (Plasmid-EZ) is our end-to-end solution for cost-effective, rapid, and high-throughput sequencing of entire plasmids. Unlike conventional methods that only sequence selected regions, Plasmid-EZ verifies the integrity and fidelity of whole plasmids using long-read sequencing. This ensures the detection of unwanted changes, providing a complete plasmid map and preventing potential replication issues.

Now connected to our expansive dropbox network, Plasmid-EZ offers unparalleled convenience for sample submission – easily order directly through our customer portal and deposit your samples at one of our convenient dropboxes. Upon receipt in our processing lab, you will receive results in as fast as one business day.

What is whole plasmid sequencing?

Whole plasmid sequencing is the comprehensive read and analysis of a plasmid’s entire genetic code, known as plasmid DNA (pDNA).

Whole plasmid sequencing provides detailed insights into the genetic makeup of plasmids and how subsequent modifications of plasmids can be used in gene knockout and editing studies. By sequencing the whole plasmid, genomic data can be used to further the understanding of microbial diversity, evolution, and mechanisms underlying biological processes.

How is whole plasmid sequencing done?

Whole plasmid sequencing is carried out by isolating the plasmid DNA (pDNA), splicing the pDNA into smaller fragments, sequencing said fragments, then assembling the plasmid sequence data to reconstruct the full plasmid genome. Bioinformatics tools are then used to analyze the resulting sequencing data.

Direct Colony to Whole Plasmid Sequencing

Simplify your research workflow and enhance your productivity with our advanced whole plasmid sequencing service (Plasmid-EZ), now accepting bacterial samples as starting material. With our experts performing plasmid preps and sequencing, we’ve streamlined our process to efficiently deliver results in as fast as 2 business days. Experience the ease and excellence of our sequencing solutions today.

PLASMID CONSTRUCTION WORKFLOW WITH PLASMID-EZ

COMPLETE PLASMID SEQUENCING WORKFLOW FOR PLASMID VALIDATION

Technology Comparison: Whole Plasmid Sequencing

Metric	Sanger Sequencing	Plasmid-EZ	Primer Walking	NGS
Starting Material	Plasmid DNA	Plasmid DNA or Bacteria*	Plasmid DNA	Plasmid DNA
Region Sequenced	Targeted Regions	Full-Length Plasmid	Long Targeted Regions	Full-Length Plasmid
Cost	$	$$	$$$	$$$
Read Quality	+++	++	+++	++++
Read Length	++	++++	++	+/++++
Automatic Assembly
Gene Annotations
Other Requirements	Must provide primer		Must provide reference

*We accept bacterial samples as colonies, liquid culture and glycerol stock

Deliverables

• FASTQ files
• Read-length and quality report
• Consensus plasmid assembly
• Plasmid annotation files

Confirm your plasmid sequences with Azenta, a worldwide leader in sequencing since 1999

Features & Benefits

Barcode Availability

Enable fully-automated workflows and traceability

Quick Turnaround Times

For rapid, complete plasmid validation

Easily Submit Samples

At any one of our convenient dropbox locations

High-throughput, plasmid-to-data solution for rapid and interactive construct validation

Same price across all plasmids, regardless of size and complexity

Low-cost, unbiased coverage for use in selection of complete, valid plasmid candidates

Flexible sample types accepted – plasmid DNA, bacterial colonies, liquid culture, and glycerol stock

Seamless and Efficient Funding

GENOMICS PREPAYMENT

Technical Resources

Blog | Ensure Complete Plasmid Validation on Your First Attempt with Oxford Nanopore Sequencing

Explore the cutting-edge realm of genomic research with Oxford Nanopore Sequencing (ONT), and how this innovative Next Generation Sequencing technology offers real-time, long-read capabilities, revolutionizing our approach to decoding genetic information.

Webinar | From Base Pairs to Breakthroughs: Understanding Sanger and Oxford Nanopore Sequencing

While Sanger sequencing remains the gold standard for accurate DNA sequencing, advancements in sequencing technology, such as Oxford Nanopore Technology (ONT), have provided a cost-effective, long-read alternative for analysis. In this workshop, delve into the considerations of each technique and discover how to interpret their resulting sequencing data effectively so you can capture the benefits of both approaches for your specific needs.

Blog | Troubleshooting DNA Templates with Sanger Sequencing

Sanger sequencing is highly effective for testing small targeted genomic regions and for validating results from NGS. However, as with all sequencing technologies, there are limitations and needs for troubleshooting. Read on to learn about the three basic troubleshooting steps we recommend to start with.

Tech Note | Optimizing Plasmid Preparation to Increase Yield and Reduce Endotoxins

Plasmid DNA (pDNA) is vital for a range of biopharmaceutical and biotechnological applications but maximizing yield while minimizing endotoxins can be challenging. This tech note explores strategies to optimize bacterial growth conditions, enhance lysis, and refine purification protocols to boost pDNA yield and purity, ensuring consistent, high-quality results.

NGS Platforms

Azenta Life Sciences is a service provider of cutting-edge NGS platforms. For information on our NGS platforms and recommended configurations of your projects, please visit the NGS Platforms page. Azenta does not guarantee data output or quality for sequencing-only projects.