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Our Barcodes for Sequencing service provides a seamless, prepaid solution for Sanger, plasmid, and amplicon sequencing needs. Leveraging our extensive experience and advanced automation technology, we offer barcodes that streamline the sequencing process, minimizing errors and enhancing efficiency.
This fast, reliable, and convenient service is designed to optimize your experience using our fully automated workflows that ensure precise sample assignment, convenient online order tracking, and secure data retrieval directly from your CLIMS account.
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Pre-Mixed/Pre-Defined Tubes Standard |
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Pre-Mixed/Pre-Defined Tubes Express |
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Pre-Mixed/Pre-Defined Plates Standard |
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Primers |
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Plasmid-EZ |
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Amplicon-EZ
USE BARCODES AS A CONVENIENT PAYMENT OPTION
Our Barcodes for Sequencing service provides handy sample labeling and a more convenient payment option for all sequencing products submitted in tubes or plates. Barcode labels can easily be shared with colleagues and managed through the Payment Center within CLIMS, our online ordering/data management system.
We provide two types of barcode options at GENEWIZ:
1. Prepaid Barcodes: Secure flexibility, convenient logistics, automated sample processing workflows, and easy online order tracking throughout the entire process. Enjoy minimized paperwork and simplified invoicing, boosting your sequencing efficiency.
2. Non-Prepaid Barcodes: Use these with any of our sequencing services for a flexible selection of your specific needs. Activate Non-Prepaid Barcodes in your CLIMS account to choose and utilize the desired sequencing service.
Our barcoding service is designed to optimize your experience by streamlining sample management and enhancing workflow efficiency. Simply order the barcodes from your CLIMS account and submit samples at your nearest dropbox or via a local courier.
Avoid delays in your Sanger data delivery by reviewing our instructions for barcode labeling.
Barcodes for Sequencing in Tubes Pre-Mixed or Pre-Defined Standard
White Barcode labels for tubes can be used for Pre-Mixed or Pre-Defined sample types submitted in tubes with Standard delivery time. Your data will be ready for download the next business day after sample receipt at Azenta. Choose between Pre-Mixed or Pre-Defined service types when placing your order on your CLIMS account and then select between Prepaid or Non-Prepaid payment options.
Barcodes for Sequencing in Tubes Pre-Mixed or Pre-Defined Express
Use blue barcodes labels for tubes for Pre-Mixed or Pre-Defined sample types submitted in tubes for Express delivery. Your data will be ready for download the next business day by 12 pm after sample drop off at your Azenta dropbox. You can choose between Pre-Mixed or Pre-Defined service type and select Prepaid or Non-Prepaid payment option. Note: For the Express delivery service, you will need to use a nearby Azenta dropbox with free pick-up at your location. Alternatively you will be provided with a DHL Express shipping kit.
Please contact sanger.europe@azenta.com if you want to request a Azenta dropbox or DHL Express shipping kit. For UK customers, please refer to Azenta's UK logistic solutions.
Barcodes for Sequencing in Plates Pre-Mixed / Pre-Defined Standard
Benefit from cost-effective Sanger high-throughput sequencing in 96-well plates available for Pre-Mixed or Pre-Defined service with Standard Delivery.White barcode labels for plates can be ordered in your CLIMS account by choosing either the Pre-Mixed or Pre-Defined service type and then selecting your preferred payment option: prepaid or non-prepaid. For the Pre-Mixed Plate Sequencing Service, please provide your purified template (DNA) with primer mixed in the same plate well according to our Sample Submission Guidelines.
For Pre-Defined Plate Sequencing Services, send one 96-well plate with 96 templates to be sequenced with one primer (96 sequencing reaction). Or send us your template (DNA) to be sequenced with different primers per one 96-well plate (for e.g 96 reactions with primer A, 96 reactions with primer B, etc)
Note: Express delivery for Sanger sequencing Plates is only available with Pre-Mixed service type. Your data will be ready for download the next business day by 12 pm after sample drop-off at your Azenta dropbox or sample collection by DHL Express. For UK customers, please refer to Azenta's UK logistic solutions.
Primer Barcodes
If you wish to use a custom primer, use purple Primer Barcodes to label your primer tube for the Pre-Defined Tube or Pre-Defined Plate Sequencing Service. If you want to use a free Azenta Universal Primer or would like Azenta to synthesize a primer for you, please define the Primer you want to use for your sequencing reaction when you place your Sanger sequencing order on your CLIMS account.
Barcodes for Plasmid-EZ, Whole Plasmid Sequencing
Use our yellow barcode labels for prepaid whole plasmid sequencing. (Non-prepaid orders for Plasmid-EZ submissions in tubes do not require barcodes). Your data will be ready for download the next business day after receipt of samples at our processing labs. Please see the Plasmid-EZ webpage for more information.
Barcodes for Amplicon-EZ
Use our orange barcode labels for prepaid amplicon sequencing. Your data will be ready for download within 8 business days upon receipt of samples at our processing labs. Please visit the Amplicon-EZ webpage for more information.
Virtual Barcodes for Sequencing Pre-Defined Vario Plate:
Barcode-driven automated workflows enable flawless and convenient sample processing. For Vario plate, digital barcodes will be deposited into your account - your Vario Plate will be labeled with a physical label at our Azenta lab.
Pre-Defined Vario Plate Sequencing Service is for sequencing plasmids or PCR fragments in 96-well plates and is our most flexible service in terms of the number of templates and the number of primers per template that can be submitted using a 96-well plate.
For each template submitted in a well of a Vario Plate, you can send / select up to four primers that are used for the sequencing of your template.
The invoicing occurs reaction-based instead of plate-based.
Features & Benefits of Barcodes for Sanger Sequencing
Speed: Fully-automated workflow processes, quick turnaround times
Convenient: Easy online ordering with no need for submitting complicated order forms
Accurate: Reliable way to accurately read encoded information, eliminating the possibility of human error
Secure: Safe and secure data download from your CLIMS account
TECHNICAL RESOURCES
Blog | NGS, qPCR, dPCR, or Sanger Sequencing: An Assay Selection Guide
Today’s molecular biologists have many options to interrogate their genome or transcriptome of interest. Next generation sequencing (NGS), quantitative PCR (qPCR), digital PCR (dPCR), and Sanger sequencing are used widely in genomics; however, choosing the best tool for your project isn’t always intuitive. To learn more about the method best suited for your project goals, use our interactive selection guide as well as the practical information about PCR + Sanger, qPCR, dPCR, and NGS approaches below.
Blog | Troubleshooting DNA Templates with Sanger Sequencing
Sanger sequencing is highly effective for testing small targeted genomic regions and for validating results from NGS. However, as with all sequencing technologies, there are limitations and needs for troubleshooting. Read on to learn about the three basic troubleshooting steps we recommend to start with.
Blog | Ensure Complete Plasmid Validation on Your First Attempt with Oxford Nanopore Sequencing
Explore the cutting-edge realm of genomic research with Oxford Nanopore Sequencing (ONT), and how this innovative Next Generation Sequencing technology offers real-time, long-read capabilities, revolutionizing our approach to decoding genetic information.
Webinar | From Base Pairs to Breakthroughs: Understanding Sanger and Oxford Nanopore Sequencing
While Sanger sequencing remains the gold standard for accurate DNA sequencing, advancements in sequencing technology, such as Oxford Nanopore Technology (ONT), have provided a cost-effective, long-read alternative for analysis. In this workshop, delve into the considerations of each technique and discover how to interpret their resulting sequencing data effectively so you can capture the benefits of both approaches for your specific needs.
Blog | Analyzing Sanger Sequencing Data
The output for Sanger sequencing is typically a chromatogram, also known as a trace or ab1 file, and a text-based sequence file. Although the latter may seem to hold all the relevant information—after all, the point of sequencing is to get a sequence—the former can't be ignored. Here, we provide a guide to understanding Sanger sequencing data.
Sanger Sequencing Quick Tips Guide
Learn from the Sanger experts how you can get the most of your DNA Sequencing. Whether you're troubleshooting a tough sequencing reaction, perfecting PCR visualization, or learning to clean up and refine your PCR products, these resources are designed to help you get the best results.
• Volume 1: Producing Robust, Single-band PCR Product
• Volume 2: PCR Clean-up of Single-band PCR
• Volume 3: Salvaging Nonspecific PCR Products
• Volume 4: PCR Visualization by Gel Electrophoresis
• Volume 5: Troubleshooting a Bad Sequencing Reaction from a PCR Template
HOW TO ORDER
Email EU: sanger.europe@azenta.com | Phone: +49 (0)341 520 122-41
Email UK: genomics.service.uk@azenta.com | Phone: +44 (0) 1279 873837
GENEWIZ from Azenta Life Sciences
European GENEWIZ Headquarters
GENEWIZ Germany GmbH, Bahnhofstrasse 86, 04158 Leipzig | +49 (0)341 520 122-41