US
Europe (English)
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JapaneseAZENTA SANGER SEQUENCING SERVICES
Sanger sequencing at Azenta Life Sciences, formerly GENEWIZ is easy and straight-forward. Our Sanger sequencing products are designed for a variety of starting materials submitted in tubes or plates. Our high-quality results, industry-leading customer service and fast turnaround times at competitive prices make us the partner of choice for academic, pharmaceutical, biotechnology, and government institutions. Sequencing results can be delivered online within hours, depending from service ordered.
Service & Options for Sanger Sequencing
Azenta offers two service types for Sanger sequencing of PCR products or plasmids: Pre-Mixed or Pre-Defined, both available for tubes and plates. Our barcode-driven automated workflows enable flawless and convenient sample processing. To determine the service that is best for your samples, please see the table and the descriptions for each service below. To ensure the best results, please submit your samples according to our Sample Submission Guidelines for each service type.
If you need help choosing your optimal Sanger service option, please contact us:
Email: sanger.europe@azenta.com | Phone: +49 (0) 341 520 122-41
Email: genomics.service.uk@azenta.com | Phone: +44 (0) 1279 873837
| Service & Options |
Pre-Mixed |
Pre-Defined |
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| Tube or Plate/Sanger-EZ |  |
 |
 |
 |
 |
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| Payment Options |
Non- Prepaid | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | |
| Prepaid | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | |
| Turnaround Time |
Standard* (White Barcode Label) |
✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
✓ Only digital barcodes |
| Next business day after sample receipt at Azenta | ||||||||
|
Express** (Blue Barcode Label) |
✓ | ✓ | ✓ | ✓ | ✓ |
✓ Only digital barcodes |
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| Next business day morning (by 12pm) after sample drop off | Next business day morning (by 12pm) after sample drop off | Next business day morning (by 12pm) after sample drop off | Next business day morning (by 12pm) after sample drop off | Next business day morning (by 12pm) after sample drop off | N/A | Next business day morning (by 12pm) after sample drop off | ||
| Starting Material | Purified template mixed with primer | PCR Product/ Plasmid | ||||||
| Deliverables | fas, ab1 | fas, ab1 | fas, ab1 | fas, ab1 | fas, ab1 | fas, ab1 | fas, ab1 | |
| Additional Services | PCR Purification |
✓ Not with Express delivery |
✓ Not with Express delivery |
✓ Not with Express delivery |
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|
Plasmid Prep / RCA (Template: bacterial colonies / glycerol stocks) |
✓ |
✓ Not with Express delivery |
✓
Not with Express delivery |
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*Terms and Conditions apply to turnaround times. Availability of Express Service Priority can be enquired during your Sanger CLIMS order. If “Express” is not available as a Service Priority option, please contact us at logistics.europe@azenta.com. Our logistics team will verify whether the Express Service Priority can be delivered for your location. For UK customers, regional variations in turnaround times also apply. Please speak to your Sales Representative for more information. |
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Pre-Mixed Tube
For sequencing plasmids or purified PCR products, this service is the most economical choice. You provide your template and primer mixed in the same tube.
Pre-Defined Tube
You submit your template in a tube and we will mix template and a defined primer when your samples are received. You can choose from different available FREE Universal Primers or provide your own, labeled with a Purple, primer barcodes
Pre-Mixed Plate
Suitable for purified templates, this service is available for high-throughput Sanger sequencing of 96 samples. You provide your template and primer mixed in the same plate well.
Pre-Defined Plate
This service is for sequencing plasmids or PCR fragments in 96-well plates and is our most economical service when sequencing a large number of samples. You provide your template and your primer separately.
Pre-Mixed Vario Plate
You provide your template and primer mixed in the same plate well, according to our Sample Submission Guidelines, and the invoicing occurs reaction-based instead of plate-based. With Vario plate you will receive digital barcodes only - your Vario plate will be labeled with an appropriate label at our lab.
Pre-Defined Vario Plate
Our most flexible service in terms of the number of templates and the number of primers per template that can be submitted using a 96-well plate. You provide your template and your primer separately and the invoicing occurs reaction-based instead of plate-based.
Order Now | Read FAQs | Sample Submission Guideline
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Features & BENEFITS
Speed
Fully-automated workflow processes, quick turnaround times
Convenient Logistics
Easy online ordering with no need for submitting complicated order forms
Accurate
Reliable way to accurately read encoded information, eliminating the possibility of human error
Secure
Safe and secure data download from your CLIMS account
TECHNICAL RESOURCES
Sanger Sequencing Quick Tips Guide
Learn from the Sanger experts how you can get the most of your DNA Sequencing. Whether you're troubleshooting a tough sequencing reaction, perfecting PCR visualization, or learning to clean up and refine your PCR products, these resources are designed to help you get the best results.
• Volume 1: Producing Robust, Single-band PCR Product
• Volume 2: PCR Clean-up of Single-band PCR
• Volume 3: Salvaging Nonspecific PCR Products
• Volume 4: PCR Visualization by Gel Electrophoresis
• Volume 5: Troubleshooting a Bad Sequencing Reaction from a PCR Template
Blog | Troubleshooting DNA Templates with Sanger Sequencing
Sanger sequencing is highly effective for testing small targeted genomic regions and for validating results from NGS. However, as with all sequencing technologies, there are limitations and needs for troubleshooting. Read on to learn about the three basic troubleshooting steps we recommend to start with.
Blog | Ensure Complete Plasmid Validation on Your First Attempt with Oxford Nanopore Sequencing
Explore the cutting-edge realm of genomic research with Oxford Nanopore Sequencing (ONT), and how this innovative Next Generation Sequencing technology offers real-time, long-read capabilities, revolutionizing our approach to decoding genetic information.
Webinar | From Base Pairs to Breakthroughs: Understanding Sanger and Oxford Nanopore Sequencing
While Sanger sequencing remains the gold standard for accurate DNA sequencing, advancements in sequencing technology, such as Oxford Nanopore Technology (ONT), have provided a cost-effective, long-read alternative for analysis. In this workshop, delve into the considerations of each technique and discover how to interpret their resulting sequencing data effectively so you can capture the benefits of both approaches for your specific needs.
Blog | Analyzing Sanger Sequencing Data
The output for Sanger sequencing is typically a chromatogram, also known as a trace or ab1 file, and a text-based sequence file. Although the latter may seem to hold all the relevant information—after all, the point of sequencing is to get a sequence—the former can't be ignored. Here, we provide a guide to understanding Sanger sequencing data.
HOW TO ORDER
Email EU: sanger.europe@azenta.com | Phone: +49 (0)341 520 122-41
Email UK: genomics.service.uk@azenta.com | Phone: +44 (0) 1279 873837
GENEWIZ from Azenta Life Sciences
European GENEWIZ Headquarters
GENEWIZ Germany GmbH, Bahnhofstrasse 86, 04158 Leipzig | +49 (0)341 520 122-41