Gene Fragments
Gene Fragments are double-stranded, linear DNA fragments of 100-3,000 bp, which are a convenient option due to their faster delivery time, lower cost, and greater flexibility. These fragments are synthesized with the same industry-leading proprietary technology that GENEWIZ from Azenta has developed for standard gene synthesis products. We provide Gene Fragments in a dried form that can be easily re-suspended, cloned, and screened to identify the correct clone for downstream applications.
More variants. Faster iteration. Rapid engineering.
Gene Fragment Turnaround Time
FragmentGENE
| Sequence Length* | Deliverable Yield | Completion Time** | Predicted Cloning Accuracy |
|---|---|---|---|
| 100-500 bp | 500 ng | 2-4 business days | >85% |
| 501-750 bp | 500 ng | 3-5 business days | >85% |
| 751-1,000 bp | 1,000 ng | 3-5 business days | >85% |
| 1,001-1,500 bp | 1,000 ng | 4-6 business days | >85% |
| 1,501-2,250 bp | 1,000 ng | 6-8 business days | >85% |
| 2,251-3,000 bp | 1,000 ng | 6-8 business days | >85% |
*Only sequences with overall GC content between 20% and 80% qualify for Gene Fragments service. GC/AT rich or sequences with complex repeats do not qualify.
**Orders containing more than 50 fragments will require additional turnaround time.
**Orders typically require an additional 2-3 BD shipping from our production facilities.
Gene Fragment Synthesis Features and Benefits
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Cloning accuracy: More than 85% of the recombinant colonies from cloning Gene Fragments will contain your desired insert
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Easy gene assembly: Easily assemble multiple gene fragments to create a larger construct for downstream applications
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Industry-leading turnaround time: Fast turnaround time starting in just a few days to meet your research deadlines
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Affordable: Very cost-effective to meet your strict budget constraints
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Quality control: Stringent quality control process to ensure high sequence fidelity
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Ease of use: GENEWIZ Sanger sequencing, with an extensive worldwide network of dropboxes, makes identifying the correct clones faster and more convenient
How are gene fragments made?
Construction begins with the base-by-base synthesis of oligonucleotides (oligos), followed by assembly into double-stranded DNA (dsDNA) fragments. These custom DNA fragments can be used directly, cloned into vectors, or assembled into larger constructs to serve a variety of research uses.
Gene Block Quality Control
- Size verification by gel electrophoresis
- Sequence confirmation via Sanger sequencing
Gene Fragment Synthesis Applications
- Antibody discovery at scale: CDR mutagenesis, affinity maturation, humanization panels
- Assemble multiple gene fragments to make full-length gene constructs for protein expression and purification
- gRNA expression cassettes for CRISPR/Cas9-based gene editing
- Donor constructs for gene editing experiments
- Template for in vitro transcription
- High-throughput screening: barcoded fragments, reporter cassettes, assay controls
- Protein engineering: linkers/tags/fusions, alanine/tiling scans, domain swaps
- Alanine/tiling mutagenesis to map function and liability
- CDR shuffling & combinatorial libraries for scFv/Fab/IgG
- Display library inserts: yeast, phage, mammalian
- Reporter & MPRA payloads for promoter/UTR tuning
- Barcoded controls & spike-ins for pooled NGS readouts
- HDR donors & landing pads for targeted integration
Gene Fragment Deliverables
Standard deliverables include 500-1,000 ng of dried double-stranded DNA, depending on sequence length.