Next Generation Sequencing: Amplicon-EZ

Amplicon sequencing made easy!

Easy on your budget – as low as $58 per sample
Easy to order – checkout online in minutes
Easy to submit samples – use a Azenta Life Sciences, formerly GENEWIZ drop box
Easy to analyze data – interactive reports with intuitive design
Easy on your deadline – results in 8 business days*

Amplicon-EZ is a streamlined version of next generation sequencing to analyze heterogeneous PCR products. Our standardized workflow simplifies ordering, processing, and analysis to deliver high-quality results fast.

Amplicon Sequencing in 8 Days*

1. Sample submission

No minimum sample number
Convenient sample submission via dropboxes

2. Library Preparation

150-500 bp products accepted
Submit with or without lllumina® partial adapters

3. Sequencing

-50,000 reads per sample
Full sequence coverage with 2x2S0 bp sequencing

4. Data Analysis

Interactive variant report
Unique sequence identification and abundance analysis

*Samples are processed at our New Jersey, USA facility in cycles starting on Wednesdays with data returned 8 business days later (on the second Monday).

Applications: CRISPR SCREENING & MORE

CRISPR screening and validation – on/off-target mutations

Somatic variant analysis

Taxonomic identification – 16S, 18S, or ITS regions

Antibody discovery – variable regions of mAb, Fab, scFv, or sdAb genes

Clone verification and screening

Diversity analysis – synthetic DNA libraries

CRISPR Screening with Amplicon-EZ

1. Edit cells with CRISPR

2. Amplify target region from edited cell populations

3. Use Amplicon-EZ to expedite sequencing of PCR products

4. Analyze results to find wells with desired mutations**

CRISPR Validation with Amplicon-EZ

1. Isolate clones from postitive wells (see CRISPR screening)

2. Amplify target region from clones

3. Use Amplicon-EZ to expedite sequencing of PCR products

4. Validate edits and find homozygous mutants**

**View our interactive report (login required)

Want Azenta to perform amplification and sequencing? Learn about our additional NGS-based CRISPR validation services.

Technical Resources

NGS PLATFORMS

For information on our NGS platforms as well as recommended configurations of your projects, please visit the NGS Platforms page. Azenta does not guarantee data output or quality for sequencing-only projects.

PacBio

10x Genomics

NanoString

Nanopore

Olink

Related Services

How To Order

Email | Phone 1-877-436-3949, Ext. 1