16S rRNA: Let GENEWIZ Help Construct Your Phylogenies

The 16S ribosomal RNA subunit is an essential component in the 30S ribosomal complex in prokaryotes. Due to the structural importance of this gene, the pace of evolution of the gene has been relatively slow. Due to this conservation, scientists can use the 16S rRNA gene to identify prokaryotic species present in a sample. GENEWIZ sequences the 16S rRNA gene from your bacterial colonies rapidly and effectively. Simply submit your clonal bacterial sample, and GENEWIZ will perform PCR amplification and Sanger DNA sequencing using 16S primers. Save time at the bench - no bacterial gDNA extraction is required.

16S rRNA Gene Sequencing Service Details

• PCR amplification directly from clonal bacterial colonies
• Enzymatic purification of crude PCR product for enhanced sequencing quality
• Additional analysis of PCR product by gel electrophoresis available by request
• Forward and reverse sequencing of the 16S rRNA gene
• Starting materials accepted:

• Clonal Bacterial Colonies
• Extracted gDNA